Table 2. Purification of PHB depolymerase of Stenotrophomonas sp. RZS7 on Octyl sepharose column.
Fraction | Total activity (UmL-1) | Total protein (mgmL-1) | Specific activity (U mg-1mL-1) | % activity |
---|---|---|---|---|
1 | 0.027 (0.027) | 0.031 (0.010) | 0.0782 (0.032) | 11.13 (0.012) |
2 | 0.101 (0.011) | 0.153 (0.037) | 0.3781 (0.041) | 48.31 (0.039) |
3 | 0.247 (0.015) | 0.309 (0.074) | 0.7993 (0.090) | 79.93 (0.092) |
4 | 0.017 (0.001) | 0.015 (0.009) | 0.0106 (0.011) | 04.01 (0.012) |
5 | 0.003 (0.001) | 0.006 (0.005) | 0.0011 (0.008) | 00.10 (0.002) |
Values were statistically significant at P ≤ 0.05
Values in the parenthesis are standard deviation