Table 3. Qualitative measurement of biodegradation of PHB film.
| Weight of PHB film | Weight loss of PHB film (g) | OD | Crotonic acid* (μgmL-1) |
|---|---|---|---|
| Initial weight | 0.159 (0.031) | 0.000 | 1000 0.030) |
| After 2 days | 0.148 (0.029) | 0.014 | 813 (0.026) |
| After 4 days | 0.109 (0.021) | 0.019 | 794 (0.019) |
| After 6 days | 0.099 (0.017) | 0.035 | 705 (0.018) |
| After 8 days | Fragmentation | 0.050 | 686 (0.015) |
Values were statistically significant at P ≤ 0.05
Values in the parenthesis are standard deviation
*Crotonic acid was as PHB standard for spectrophotometric estimation of PHB as when PHB is heated with concentrated sulphuric acid it gets converted into crotonic acid.