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Blocking IL6 can reverse partial of the role Polyphyllin I in PC cells in the expression of P21. Western blot results confirmed that blocking IL6 could reduce the upregulation of P21 in protein level both in PC3 (A) and DU145 cells (B). RT-PCR assay confirmed that blocking IL6 could reduce the upregulation of P21 in mRNA level both in PC3 (C) and DU145 cells (D). RT-PCR = reverse transcription-polymerase chain reaction.
