Skip to main content
. 2019 Dec 9;8:e50094. doi: 10.7554/eLife.50094

Figure 3. IRES-containing mRNAs are more efficiently associated with polysomes in hypoxic conditions.

(A, B) RQ values for the IRES-containing mRNA transcriptome (A) and translatome (B) extracted from the PCR arrays shown in Figures 1 and 2. The gene Aplnr (apelin receptor) was chosen as a control without an IRES. Vegfc and Fgf2 mRNAs, which are repressed in the transcriptome, were below the detection threshold in polysomes (ND). Histograms correspond to means ± standard deviation, with values for hypoxia compared to those for normoxia by a bilateral Student's test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Figure 3.

Figure 3—figure supplement 1. Transcriptome of IRES-containing mRNAs in hypoxic cardiomyocytes.

Figure 3—figure supplement 1.

RQ values for IRES-containing mRNA transcriptome kinetics extracted from the PCR arrays shown in Supplementary file 1. The gene Aplnr (apelin receptor) was chosen as a control without an IRES. Relative quantification (RQ) of gene expression in hypoxia was calculated using the 2–ΔΔCT method with normalization to 18S and to normoxia. Standard deviation is indicated. When the RQ value is inferior to 1, the fold change is expressed as −1/RQ. ND means ‘non detected’.