Figure 8. VASH1 depletion has both activating and inhibiting effects on mRNA recruitment into polysomes.

HL-1 cardiomyocytes were treated with siVASH1 or siControl and submitted to 8 hr of hypoxia, or maintained in normoxia. RNA was purified from polysome fractions and from cell lysate before loading. cDNA and PCR arrays were performed as in Figure 1. Relative quantification (RQ) of gene expression during hypoxia was calculated using the 2^–ΔΔCT method with normalization to 18S rRNA and to SiControl. mRNA levels (polysomal RNA/total RNA) are shown as fold change of repression (red) or induction (green) in siVASH1 cells normalized to SiControl-treated cells. Non-regulated mRNAs are represented in blue. The threshold for induction was set at 1.5. When the RQ value is inferior to 1, the fold change is expressed as −1/RQ. The detailed values are available in Supplementary file 7.