Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (firefly, Photinus) | Luc+ | Promega | Modified firefly luciferase | |
| Gene (Renilla reniformis) | LucR | Promega | Renilla luciferase | |
| Strain, strain background (Escherichia coli) | TOP 10 | Thermofisher Scientific | Transformation-competent cells (genotype : F– mcr A Δ (mrr –hsd RMS–mcr BC) φ 80lac ZΔ M15 ΔlacX 74 rec A1 ara D139 Δ (araleu) 7697 gal U gal K rps L (StrR) end A1 nup G) |
|
| Cell line (Homo sapiens) | 293FT | Invitrogen | R700-07 | High-transfection performance for lentivector production |
| Cell line (Homo sapiens) | HT1080 | ATCC | CCL-121 | |
| Cell line (Mus musculus) | HL-1 | William C. Claycomb (Claycomb et al., 1998) | Cardiomyocyte cell line with beating phenotype | |
| Recombinant DNA reagent | pTRIP-CRHL+ | Dryad, Supplementary file 8A (Morfoisse et al., 2014) |
Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by an intergenic palindromic sequence | |
| Recombinant DNA reagent | pTRIP-CRF1AL+ | Sequence available on Dryad, (Martineau et al., 2004; Ainaoui et al., 2015) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human FGF1 IRES A | |
| Recombinant DNA reagent |
pTRIP-CRFL+ | Sequence available on Dryad, (Créancier et al., 2000) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human FGF2 IRES | |
| Recombinant DNA reagent | pTRIP-CRVAL+ | Sequence available on Dryad, (Huez et al., 1998) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human VEGFA IRESa | |
| Recombinant DNA reagent |
pTRIP-CRVBL+ | Sequence available on Dryad, (Huez et al., 1998) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human VEGFA IRESb | |
| Recombinant DNA reagent | pTRIP-CRhVCL+ | Sequence available on Dryad, (Morfoisse et al., 2014) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human VEGFC IRES |
|
| Recombinant DNA reagent | pTRIP-CRhVDL+ | Sequence available on Dryad, (Morfoisse et al., 2016) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human VEGFD IRES | |
| Recombinant DNA reagent | pTRIP-CRMP2L+ | Sequence available on Dryad, (Nanbru et al., 1997) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the human c-myc IRES | |
| Recombinant DNA reagent |
pTRIP-CREL+ | Sequence available on Dryad, (Créancier et al., 2000) | Bicistronic SIN lentivector construct with the CMV promoter controlling expression of LucR and Luc+ separated by the EMCV IRES | |
| Recombinant DNA reagent | pCMV-dR8.91 | Addgene | Trans-complementing plasmid containing the lentiviral protein genes (gag, pol, rev…) | |
| Recombinant DNA reagent | pCMV-VSV-G | Addgene | #8454 | Trans-complementing plasmid containing VSV G envelope protein gene |
| Transfected construct (mouse) | Acell SMARTpool targeting VASH1 (siVASH1) | Dharmacon | siRNA targeting VASH1 (Supplementary file 8B) | |
| Transfected construct (mouse) | Control SMARTpool | Dharmacon | Scramble siRNA (Supplementary file 8B) | |
| Sequence-based reagent | Deltagene assay | Fluidigm Corporation, oligonucleotide sequences available in Supplementary file 6 | PCR array with 96 oligonucleotides primer couples | |
| Antibody | Mouse monoclonal anti-VASH-1 | Abcam | EPR17420 | Jess Western 1:100 |
| Antibody | Mouse monoclonal anti-VASH-1 | Abcam | ab176114 | IC 1:50 |
| Antibody | Mouse monoclonal anti-FGF1-1 | Abcam | EPR19989 | Jess Western 1:25 |
| Antibody | Mouse monoclonal anti-p21 | Santa Cruz | sc-6546 (F5) | Jess Western 1:10 |
| Antibody | Rabbit polyclonal anti eIF2α | Cell Signaling Technology | 9721 | Jess Western 1:50 |
| Antibody | Mouse monoclonal anti-phospho-eIF2α | Cell Signaling Technology | 2103 | Jess Western 1:50 |
| Antibody | Rabbit polyclonal anti-4EBP-1 | Cell Signaling Technology | 9452 | Jess Western 1:50 |
| Antibody | Rabbit polyclonal anti-phospho-4EBP-1 | Cell Signaling Technology | 9451 | Jess Western 1:50 |
| Antibody | Secondary-HRP (ready to use rabbit ‘detection module’) | Protein Simple | DM-001 | Jess Western |
| Antibody | Donkey anti-rabbit alexa488 | Jackson Immunoresearch | 711-545-152 | Secondary antibody for IC |
| Software, algorithm | PRISM | Graphpad | Statistical analysis |