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. 2019 Nov 26;13:979. doi: 10.3332/ecancer.2019.979

Table 1. Summary of the reported papers on CA15-3 levels during normal and pathological pregnancies.

Authors/year of publication Pathological conditions of pregnancy Sample Number of patients Cut-off value (U/mL) Results
Touitou et al, 1989 [11] None MS - 100 healthy pregnant women
- 22 healthy non-pregnant volunteers
<25 Major increase during the third trimester; none above cut-off value.
Lelle et al, 1989 [25] Pre-eclampsia UC, MS and AF 134 pregnant women at 7–43 weeks of gestation (3/134: twin pregnancies; 5/134 pre-eclampsia) £25 9.4% of CA 15-3 values were above the cut-off during gestation.
Not detectable in UC.
Low in AF with a range of 2–18 U/mL.
Schrocksnadel et al, 1993 [10] Gestational hypertension MS - 50 patients with gestation hypertension
- 50 healthy pregnant patients
- 50 healthy non-pregnant controls
£25 CA 15-3 lower in non-pregnant con­trols (median < 5 U/L) than in healthy pregnant women
(median 21 U/L) (p < 0.0001), but similar in healthy and hypertensive pregnant women (median 18 U/L)
(p = 0.34)
Noci et al, 1995 [22] Down’s
syndrome
AF - 20 single Down’s syndrome pregnancies at 15–19 weeks
- 60 controls
NR The median MoM values of CA 15-3 in Down’s syndrome pregnancies were 1.16 MoM, not significantly different from those of unaffected pregnancies (CA 15.3: 0.99 MoM).
Schlageter
et al, 1998 [12]
None MS 12 healthy pregnant women at 6–40 weeks of amenorrhoea £30 Linear temporal evolution of CA1 5-3 but with concentrations in the usual range of values.
Tayyar et al, 1999 [17] None AF and MS 62 pregnant women at 16–20 weeks of
gestation
NR MS CA 15-3 values were elevated in the
primigravida group.
Cheli et al, 1999 [13] None MS 90 healthy pregnant women during the three trimesters of pregnancy NR CA 15-3 values were above the cut-off (3.3%) and were significantly elevated in the third trimester as compared to the first trimester of pregnancy (p < 0.05).
Botsis et al, 1999 [16] None AF and MS - T1 = 20;
- T2 = 29;
- T3 = 26;
- at parturition = 20;
- controls: 20 healthy, age-matched, non-pregnant women
£33 CA 15-3 values in AF, which were marginally higher than in MS, did not differ significantly with the progression of pregnancy. 5%, 10% and 20% above cut-off value, in the three trimesters, respectively.
Bon et al, 2001 [18] Spontaneous abortion, foetal death, intrauterine growth retardation, chromosomal abnormalities, (pre)-eclampsia and structural abnormalities MS - 350 normal pregnancies (T1 = 127, T2 = 192, T3 = 47)
- 120 pathological pregnancies
(spontaneous abortion = 8, foetal death = 8, intrauterine growth retardation = 46, chromosomal abnormalities = 12, (pre)-eclampsia = 6 and structural abnormalities = 42)
NR MS CA 15-3 levels in normal pregnancies were significantly higher during the third trimester compared to the first two trimesters of pregnancy.
Serum CA15-3 levels in the serum of pregnant women with a pathological outcome were not significantly different from those found in normal pregnancy.
Hegab et al, 2003 [20] Complete
hydatidiform mole
MS - 60 cases of complete hydatidiform mole
- Controls: 20 therapeutic abortion of a corresponding duration of pregnancy
NR No significant statistical difference was found between all groups.
Kiran et al, 2005 [14] No UC and MS 53 pregnant women just before caesarean delivery of full-termed pregnancies
(38–42 weeks)
£30 MS levels of CA 15-3 are not influenced by pregnancy.
UC CA 15-3 levels were significantly lower than MS levels at term pregnancies.
Ercan et al, 2011 [15] No MS 30 healthy pregnant women £25 It was found that the three trimesters had statistically similar levels for serum CA 15-3
(median values 17.5, 19.7 and 18.3 U/mL, respectively).
CA 15-3 assay values were found generally within the normal range.
Fourteen patients’ CA 15-3 values were elevated above the cut-off value of 25 U/mL (16%) during each trimester: T1 = 5; T2 = 5; T3 = 4.
Akinlade et al, 2012 [21] Trisomy 21 MS - 69 trisomy 21
- 388 euploid controls
NR Not affected by gestational age.
No evidence of a significant difference in CA 15–3 concentrations among trisomy 21 and control pregnancies
(1.03 MoM in euploid, 1.09 in trisomy 21,
p = 0.130)
Sharma et al, 2015 [19] Gestational
diabetes,
preexisting heart disease, intrahepatic cholestasis of pregnancy, thyroid disorder, anaemia, coagulation disorder, hypertensive disorder, epilepsy
MS - 31 non-pregnant women
- 251 pregnant women:
77/251 (30.7%) with high risk pregnancies:
gestational diabetes = 20 (26.0%)
preexisting heart disease = 16 (20.8%)
intrahepatic cholestasis of pregnancy 13 (16.9%)
thyroid disorder = 11 (14.3%) (9 hypothyroidism and 2 hyperthyroidism)
anaemia = 11 (14.3%)
coagulation disorder = 9 (3.6%)
hypertensive disorder = 9 (3.6%)
epilepsy = 3 (3.9%)
£30 Among the pregnant women, 93 (37.1%) had a CA 15-3 value above the normal cut-off.
CA 15-3 concentration was significantly higher in the second and third trimesters than in the first trimester (p ≤ 0.01 for both comparisons).
CA 15-3 level was also increased in pregnancies associated with gestational diabetes mellitus, intrahepatic cholestasis of pregnancy and heart disease.

NR, not reported; MS, maternal serum; AF, amniotic fluid; UC, umbilical cord; T1, first trimester; T2, second trimester; T3, third trimester