Table 2. Sensitivity analysis of simplex assays for serial dilution of genomic DNA from pathogenic Cmm, Pst and Xe.

Mean Ct values are presented.

Amount of DNA per reaction	HEX (Cmm NCPPB 2797)	FAM (Pst NCPPB 1106)	CY5 (Xe NCPPB 2689)
100 ng	15.71	16.75	21.52
10 ng	19.02	19.49	24.53
1 ng	22.55	23.10	26.94
0.1 ng	25.61	26.09	31.82
0.01 ng	29.11	30.36	33.50
0.001 ng	-	33.31	-
Efficiency (%)	99.3	93.4	99.6
Slope	-3.340	-3.490	-3.331
Y-intercept	22.40	22.99	27.87