Table 3. The mean Ct values for each fluorescent TaqMan® probe obtained from the designed multiplex real-time PCR assays.
Mean Ct values ± standard deviation are presented.
| Target bacteria | Isolate | HEX | FAM | Cy5 |
|---|---|---|---|---|
| C. m. subsp. michiganensis | NCPPB 515 | 20.63 ± 0.08 | - | - |
| NCPPB 1064 | 21.43 ± 0.05 | - | - | |
| NCPPB 1496 | 21.27 ± 0.04 | - | - | |
| NCPPB 2323 | 21.34 ± 0.06 | - | - | |
| NCPPB 2979* | 20.89 ± 0.01 | - | - | |
| NCPPB 3120 | 21.56 ± 0.03 | - | - | |
| P. s. pv. tomato | NCPPB 878 | - | - | - |
| NCPPB 1106* | - | 22.01 ± 0.67 | - | |
| NCPPB 2683 | - | 24.71 ± 0.15 | - | |
| NCPPB 3333 | - | 22.43 ± 0.26 | - | |
| NCPPB 3787 | - | 24.09 ± 0.62 | - | |
| NCPPB 4369 | - | 21.72 ± 0.39 | - | |
| CRI 111 | - | 23.33 ± 0.49 | - | |
| CRI 211 | 37.87 ± 1.12 | 23.69 ± 0.05 | - | |
| X. euvesicatoria | NCPPB 941 | - | - | 23.46 ± 0.25 |
| NCPPB 2574 | - | - | 23.40 ± 0.66 | |
| NCPPB 2594 | - | - | 23.03 ± 0.47 | |
| NCPPB 2968* | - | - | 23.19 ± 0.13 | |
| X. vesicatoria | NCPPB 422* | - | - | 22.51 ± 0.20 |
| NCPPB 1421 | - | - | 23.26 ± 0.84 | |
| NCPPB 2044 | - | - | 22.75 ± 0.04 | |
| NCPPB 3786 | - | - | 24.82 ± 0.45 | |
| X. gardneri | NCPPB 881* | - | - | 21.86 ± 0.51 |
| X. perforans | NCPPB 4321* | - | - | 21.97 ± 0.04 |
| X. a. pv. vesicatoria | CRI 1008 | - | - | 24.42 ± 0.13 |
| CRI 1009 | - | - | 24.52 ± 0.13 | |
| CRI 1011 | - | - | 24.38 ± 0.08 | |
| CRI 1013 | - | - | 24.21 ± 0.10 | |
| CRI 1016 | - | - | 24.23 ± 0.27 | |
| CRI 1018 | - | - | 24.39 ± 0.28 | |
| CRI 1023 | - | - | 24.54 ± 0.17 | |
| CRI 1026 | - | - | 23.77 ± 0.10 | |
| Nontarget bacteria | ||||
| C. m. subsp. insidiosus | NCPPB 1109* | - | - | - |
| C. m. subsp. tesselarius | NCPPB 3664* | - | - | - |
| P. s. pv. syringae | NCPPB 2750 | - | - | - |
| P. corrugata | NCPPB 2445* | - | - | - |
| P. c. subsp. carotovorum | NCPPB 281* | 37.07 ± 0.83 | - | - |
| X. a. pv. phaseoli | NCAIM B.01695 | - | - | 23.93 ± 0.04 |
| X. c. pv. armoraciae | NCAIM B.01281 | - | - | - |
| X. c. pv. campestris | NCPPB 528* | - | - | - |
| X. c. pv. incanae | HRIW 6377 | - | - | - |
| X. c. pv. raphani | HRIW 8503 | - | - | - |
| X. cucurbitae | NCAIM B.01397 | - | - | - |
| X. h. pv. carotae | NCAIM B.01586 | - | - | 24.89 ± 0.08 |
* Reference isolate