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. 2020 Jan 2;7(1):ENEURO.0391-19.2019. doi: 10.1523/ENEURO.0391-19.2019

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Copyright © 2020 Robison et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — A, Survival of newly born cells in the dentate gyrus, as measured by the mean (±SEM) number of EdU⁺ cells 28 d post-EdU injection throughout the whole hippocampus and in the dorsal and ventral subregions. There were no group differences in any area measured. B, Newly born cells in the dentate gyrus that differentiated into neuroblasts/immature neurons and survived 28 d post-EdU injection, were measured by the mean (±SEM) number of EdU⁺/DCX⁺ cells throughout the whole hippocampus and in the dorsal and ventral subregions. In the dorsal hippocampus, females had a greater number of EdU⁺/DCX⁺ cells compared with males, regardless of diet. C, Newly born cells in the dentate gyrus that differentiated into mature neurons and survived 28 d post-EdU injection, were measured by the mean (±SEM) number of EdU⁺/NeuN⁺ cells throughout the whole hippocampus and in dorsal and ventral subregions. There were no group differences in any area measured. N = 10/group. *p < 0.05 main effect of sex. D, Representative images of EdU (cyan) alone and colocalized with DCX (green) and NeuN (red).