Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | Emx1-Cre | Jackson Laboratory (Bar Harbor, ME). | Emx1B6.129S2-Emx1tm1(cre)Krj/J | RRID:IMSR_JAX:005628 |
| Strain, strain background (M. musculus) | Vangl2 flox/flox | Ramsbottom et al., 2014 | ||
| Recombinant DNA reagent | actin-mEos2 | Garcia et al., 2015 | ||
| Recombinant DNA reagent | N-cadherin-mEos2 | Garcia et al., 2015 | Uniprot : X07277 | |
| Recombinant DNA reagent | N-cadherin-GFP | Thoumine et al., 2006 | Uniprot : X07277 | |
| Recombinant DNA reagent | EB3-eGFP | Leterrier et al., 2011 | ||
| Recombinant DNA reagent | pEGFP-C3 | Clontech | ||
| Recombinant DNA reagent | mEos2-Vangl2 | This paper | Uniprot : Q91ZD4 | |
| Transfected construct | AAV9-hSyn1-GFP-2A-GFP-f | Given by Dr G Feng; Zhang et al J Neurosci. 2016; PMID: 26888934 | Adeno-associated virus construct to transfect and express GFP. Titer: 2.4 × 1013 gcp/ml | |
| Other | Latex microspheres | Polysciences, | ref: 19404 | |
| Peptide, recombinant protein | ChromPure Human IgG, Fc fragment | Jackson Immuno Research | ref: 009-000-008 | 0.04 mg/mL |
| Peptide, recombinant protein | Recombinant human N-cadherin Fc chimera Protein, CF | R and D Systems | Ref 1388-NC-050 | 0.04 mg/mL |
| Antibody | AffiniPure Goat anti-human IgG, Fcγ Fragment specific | Jackson Immunoresearch | Ref 109-005-098 | 0.023 mg/mL |
| Antibody | Rabbit polyclonal anti-Vangl2 | Montcouquiol et al., 2006 | 1/500 | |
| Antibody | guinea pig polyclonalanti-Doublecortin-X | Millipore | Ref AB2253 | 1/2000 |
| Antibody | Mouse monoclonal anti-β-catenin | BD Biosciences | clone 14 | 1/200 |
| Antibody | Mouse monoclonal anti-GAPDH | Millipore | Ref AB 2302 | 1/5000 |
| Antibody | Amersham ECL Mouse IgG, HRP-linked whole Ab (from sheep) | GE Healthcare UK | Ref NA931 | 1/5000 |
| Antibody | Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey) | GE Healthcare UK | Ref NA934 | 1/5000 |
| Antibody | Rabbit polyclonal anti-pan actin | Cytoskeleton | Ref AANO1 | 1/500 |
| Antibody | Mouse monoclonal anti-N-cadherin | BD Biosciences | clone 32 | 1/200 |
| Antibody | Mouse monoclonal Anti-Neurofilament-H | Hybridoma Bank | 2H3 | 1/100 |
| Antibody | Rabbit polyclonal anti-Cux1 | Santa Cruz | Ref Sc13024 | 1/200 |
| Other | Fluoromyelin | Thermo Fisher Scientific | BrainStain Imaging kit, Ref B34650 | 1/300 |
| Other | Phalloidin Coumarin | Sigma | Ref P2495 | 1/500 |
| Software | MetaMorph | Molecular Devices | ||
| Algorithm | PALM Tracer | Kechkar et al., 2013 | JB Sibarita | Custom made algorithm |
| Software, algorithm | Mathematica 4.1 version, Wolfram Research |
Czöndör et al., 2012; Garcia et al., 2015 | O Thoumine | Custom made algorithm |
| Software | Prism 6.0 | Graphpad software | ||
| Software | Hamamatsu NDP viewer | Hamamatsu |