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HT-1197 (A) and UMUC3 (B) cells were transfected with circPDSS1 expression vector or miR-16 mimic. Overexpression of circPDSS1 and miR-16 was confirmed by RT-qPCR. The effects of circPDSS1 overexpression on miR-16 and the effects of miR-16 overexpression on circPDSS1 were also analyzed by RT-qPCR. All experiments were repeated three times and mean values were presented and compared (*, P<0.05).
