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. 2020 Jan 7;12:3. doi: 10.1038/s41368-019-0069-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a qPCR analysis of ectopic overexpression of miR-203 and miR-200c in 16B/TNF cells transfected with pre-miR-203, pre-miR-200c, or a combination of both miRNAs. Scramble oligonucleotides were transfected into 16B/TNF cells as a control. The relative amount of miRNAs in 16B/TNF cells transfected with pre-miRNAs was plotted as fold induction compared to that in 16B/TNF cells transfected with the scramble control 6 days post transfection. b The effect of miR-203 and miR-200c on the self-renewal of 16B/TNF cells was determined by a tumor sphere formation assay. *P < 0.01 compared to the scramble control by two-tailed Student’s t test. c Effect of miR-203 and miR-200c on migration of 16B/TNF cells was determined by transwell migration assay. *P < 0.05. d Effect of miR-203 and miR-200c on chemoresistance of 16B/TNF cells was determined by chemosensitivity assay. Five hundred cells were seeded in 96-well plates and treated with 40 µmol·L⁻¹cisplatin (Cisp) or 25 µmol·L⁻¹ methotrexate (Meth) for 4 days. *P < 0.05 compared to scramble. e The effect of miR-203 and miR-200c on the Notch pathway was determined by Western blotting using an antibody against the activated form of Notch1 protein (NICD). f Effect of miR-203 and miR-200c on the NICD downstream target Hes-1 was determined by qPCR. *P < 0.05 compared to the scramble control.