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. 2020 Jan 7;11:52. doi: 10.1038/s41467-019-13950-4

Fig. 3. Impacts on Polyamine Biosynthesis in androgen sensitive and androgen independent lines.

Fig. 3

LAPC-4, LNCaP, C4-2, and CWR22Rv1 cells were treated with vehicle control (black bars), 1 nM MTDIA (red bars), 1 µM BENSpm (blue bars), or the combination (B + M) (green bars) for 8 days in the presence of 20 µM MTA. a Intracellular putrescine, spermidine, and spermine levels measured by UPLC for all 4 cell lines. Values are normalized to protein concentrations. b Extracellular acetylated polyamines (acetylated spermidine + acetylated spermine) extracted from the media measured by UPLC. Values are normalized to intracellular protein concentrations. c RNA expression as measured by Real-Time rt-PCR for solute carrier 3 family member 2 (SLC3A2). Values are normalized to GUSB expression and made relative to vehicle control. d Intracellular s-adenosylmethionine (SAM) to s-adenosylhomocysteine (SAH) ratio as measured by UPLC. Results for biological triplicates are shown. Statistical analyses were performed using an unpaired student t-test with Welch’s correction. All values are compared to vehicle control. Error bars represent the standard deviation of the mean. *p < 0.05, **p < 0.01, ***p < 0.001.