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. 2020 Jan 7;11:23. doi: 10.1038/s41467-019-13838-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Multiple instances of merging and splitting GFP-Rab11A foci (shown here in gray) can be identified in live-imaging datasets. a–i Chronologically ordered time snapshots follow a moving GFP-Rab11A spot merging with and splitting from other GFP-Rab11A foci. The yellow track shows location history and current direction of motion. For clarity, only centers of those GFP-Rab11A foci that take part in this interaction are denoted by red spots. The surrounding vesicles are either stalled or very slow moving. Timestamps show time elapsed since the start of the dataset. Scale bars are 0.5 μm as shown.