Fig. 4. Effect of cytoskeleton depolymerization on motion dynamics of influenza vRNP in A549 cells.

Motion parameters of influenza vRNP segments with a fluorescently tagged polymerase. A549 cells were infected with H1N1pdm PA::GFP at multiplicity of infection (MOI) = 1 and treated with drugs 4 h post infection (hpi) to allow unhindered viral entry. Imaging was performed 16 hpi at 37 °C on a temperature-controlled stage. Each datapoint represents an individual cell (DMSO: n = 10 cells, nocodazole: n = 9 cells, and latrunculin A: n = 5 cells) using CF₅₀ for the corresponding motion parameters: a track mean speed, b track mean velocity, c track straightness, and d track arrest coefficient. Image processing and analysis were performed as described in Methods. Mean ± SD are shown per group (* implies p < 0.05, comparisons performed using one-way ANOVA with Tukey’s multiple tests). Source data are provided as a Source Data file.