Fig. 5. Effect of viral infection on Rab11A motion: comparing RSV infection with IAV (WSN) infection.

A549 cells stably expressing GFP-tagged Rab11A were infected with lab-adapted H1N1 strain (A/WSN/33) at MOI = 3 to allow a majority of cells to be infected. Imaging was performed 8 hpi at 37 °C on a temperature-controlled stage. Similarly, A549 cells stably expressing GFP-tagged Rab11A were infected with recombinant RFP-expressing RSV⁵³ at 0.1 plaque-forming units (pfu)/cell and imaging performed at 16 hpi. Infection with specific strains is noted on the x-axis along with uninfected control (uninf.). Each datapoint represents an individual cell (uninfected: n = 10 cells, WSN: n = 10 cells, and RSV: n = 4 cells) using CF₅₀ for the corresponding motion parameters: a track mean speed, b track mean velocity, c track straightness, and d, track arrest coefficient. Image processing and analysis were performed as described in Methods. Mean ± SD are shown per group (* implies p < 0.05, comparisons performed using one-way ANOVA with Tukey’s multiple tests). Source data are provided as a Source Data file.