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. 2020 Jan 7;11:78. doi: 10.1038/s41467-019-13851-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative immunoblots (IB) of phospho-SPAK/OSR1 (pSPAK/pOSR1), phospho-KCC3 (pKCC3), and phospho-NKCC1 (pNKCC1) in mouse brains studied 24 h post reperfusion after ischemic stroke. Membrane protein fractions were prepared from contralateral (CL) and ipsilateral (IL) cerebral hemispheres. Vehicle (Veh, DMSO) or ZT-1a (5 mg/kg) were administered as described in Fig. 7a. Na⁺–K⁺ ATPase α-subunit served as loading control for membrane protein fraction. b Densitometry analyses of immunoblots (similar to those in panel a) of pSPAK/pOSR1, pKCC3, pNKCC1, tSPAK/tOSR1, and tNKCC1 in mouse brains studied 24 h of reperfusion after tMCAO. Data are means ± SEM, n = 6 per group (male 3, female 3). ***p < 0.001; **p < 0.01; *p < 0.05 versus control, one-way ANOVA.