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. 2020 Jan 7;3:2. doi: 10.1038/s42003-019-0727-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a d-arabinose (25 mM) enhances wild-type C. jejuni 11168 growth (gray bars) relative to growth in unsupplemented minimal essential medium alpha (white bars) and this growth advantage is lost in the key metabolic mutants of the l-fucose pathway. Values of bars represent means (n = 4 and error bars show one standard deviation). Values of individual replicates are overlaid as dots. Asterisks indicate a significant increase in growth in the presence of d-arabinose and p-values from a two-tailed paired Student’s t-test are indicated in parentheses. b C. jejuni 11168 wild-type displays chemotaxis (indicated by pink color development and + sign) to d-arabinose and this is lost in a fucX mutant strain. Similarly, C. jejuni 81–176 wildtype does not show chemotaxis to l-fucose and complementation with fucX confers this ability. l-fucose serves as a FucX-dependent control, L-serine serves as an independent positive control, and PBS serves as a negative control. c d-arabinose reduces transport of radiolabelled l-fucose into fucose-utilizing cells (11168 wildtype and 81–176Ωfuc) more than other aldoses in both uninduced (white bars) and cells pre-grown with 20 mM l-fucose (gray bars). Values represent means of two biological and two technical replicates, standard deviations are indicated by error bars, and values from individual replicates are overlaid as dots.