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. 2020 Jan 7;3:5. doi: 10.1038/s42003-019-0735-5

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Fig. 2 — a Electron micrograph focusing on the complex network of filaments as seen in Fig. 1e that make up the glycocalyx layer. b Segmentation of a tomographic volume of the three-dimensional network. c Distribution of the pore size between filaments. Mean value = 29 ± 10 nm, n = 101. d–g Tomograms showing distinct inter-filament interactions. e Sequential tomographic slices showing lateral contact (arrows) between adjacent filaments. f Sequential tomographic slices showing a contact with no resolvable separation between the filaments. g Tomogram slice view of a filament cross-section showing the platinum coating (gray in left panel, gold in right panel) encasing the segmented filament (highlighted in blue). h Distribution of filament thickness after accounting for platinum coating. Mean = 5.3 ± 1.3 nm, n = 51. i Local bending (arrowheads) of individual filaments causes warping of adjacent segments of the network, suggesting that the filaments are under tension. j Sequential slices showing a quasi-periodic substructure (arrowheads) on the surface of the replica encasing a filament as well as in the hollow core (blue), which outlines the actual filament topography (arrows). Bar: a 200 nm; d–g, i, j 20 nm.