Fig. 5.

ROS is increased in islets from both male and female offspring of obese dams but expression of antioxidant enzyme is increased only in female islets. (a, b) ROS levels measured by DCFDA fluorescence intensity following incubation with 2.8 mmol/l glucose (a) or 16.7 mmol/l glucose (b). (c, d) qRT-PCR analysis of Cat and Sod2 mRNA expression in male (c) and female (d) offspring. Experiments were performed on islets isolated from 8-week-old male and female offspring of control and obese dams. (a, b) Data were analysed by two-way ANOVA followed by a simple main effect analysis when a significant interaction was observed. (c, d) Data were analysed independently by unpaired Student’s t test (control vs obese). *p < 0.05 and **p < 0.01 as shown. (a, b) Males, control: n = 7, obese: n = 5 mice; females, control: n = 8, obese: n = 4 mice; (c, d) Cat: males, control: n = 7, obese: n = 8 mice; females, control: n = 7, obese: n = 7 mice; Sod2: males, control: n = 8, obese: n = 6 mice; females, control: n = 9, obese: n = 9 mice. ‘n’ represents the number of mice from separate litters. All data are mean ± SEM