Fig. 7.

ASA prevents tumor cell induced angiogenesis in vivo. a Diagrammatic representation of in vivo gel-foam angiogenesis assay, b Illustrative image of blood vessel formation under mouse skin in the presence or absence of tumor cell derived conditioned media without (middle panel) or with (right panel) ASA treatment. Regular media (RM) was used as control. Note, CM-induced dilated blood vessels with high magnification are shown in the inset. Scale bar, 100 μm, (c–d). Error-bar show the mean numbers of capillary-like structures in different experimental conditions. Data show mean ± SD and are representative of at least five independent animals. Note, ASA was either directly used to treat MDA-MB-231 for CM collection (c) or fed mice through milk (d)