Table 1.
Primer sequences used for RTL measurement and mRNA expression
| qPCR primers for RTL | Sequences (5′ – 3′) |
|---|---|
| Telomere† | |
| TelAFa | CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT |
| RTel2 | GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT |
| 36B4 single copy gene‡ | |
| 36B4(F) | CAGCAAGTGGGAAGGTGTAATCC |
| 36B4(R) | CCCATTCTATCATCAACGGGTACAA |
| Real time qPCR primers | Sequences (5′ – 3′) |
| Trf1 | |
| (F) | TCTAATGAAGGCAGCGGCAA |
| (R) | ACCGCAGACTGTTTGTCACTA |
| Trf2 | |
| (F) | AGAGAAGAATCCCAAAGTACCCA |
| (R) | TACTGTCTTCATCTGGTGCTG |
| Tpp1 | |
| (F) | CGGGTGTTGGTCTGTCTCTG |
| (R) | AGGTTCCTGGAAGGATGTGC |
| Tinf2 | |
| (F) | CTAAAGGCCAAGGTGGTGGT |
| (R) | CCTGCTTTGTAGCCTTGGGA |
| Rap1 | |
| (F) | CGGGAGGGGGTAGCTATTCT |
| (R) | GTCCCTCACGAACAGAGTCG |
| Pot1 | |
| (F) | TTGAGGGAACTTTGGGAGCC |
| (R) | GATGCCCAAACACGTAAGGC |
| 18S | |
| (F) | CGGCTACCACATCCAAGGAA |
| (R) | GCTGGAATTACCGCGGCT |