Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 31;13(4):549–560. doi: 10.1007/s12079-019-00522-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — miR-873-5p inhibits tumorigenesis in colorectal cancer tissues and cell lines. a The expression of pri-miR-873-5p or ASO-miR-873-5p was analyzed in HCT116 cell or SW480 cell via RT-qPCR. b Analysis of miR-873-5p on HCT116 and SW480 cellular viabilities were confirmed by MTT assay. c Colony formation ability of HCT116 and SW480 cells were confirmed by colony formation assay. d–e MiR-873-5p suppressed cell migration ability and cell invasion ability were demonstrated via transwell migration assays or transwell invasion assays. f–g Flow cytometry assay showed the cell cycle in HCT116 and SW480 cells by transfectting with pri-miR-873-5p or ASO-miR-873-5p and respective controls, and proliferation index (PI) was counted via PI = (S + G2)/G1. h The apoptosis rate was demonstrated via Flow cytometry or AV/PI double staining. i Western blot assays showed that The protein levels of E-cadherin, cytokeratin, and Vimentin in HCT116 and SW480 cells by transfectting with pri-miR-873-5p or ASO-miR-873-5p and respective controls. All the experiments were repeated more than three times.*, p < 0.05; **, p < 0.01;***, p < 0.001