Fig. 3.

miR-873-5p directly targets JMJD8 in colorectal cancer cells. a The predicted miR-873-5p binding sites using TargetScan 7.1 in JMJD8 mRNA 3’UTR or the mutational 3’UTR of JMJD8 mRNA were confirmed. b–c HCT116 and SW480 cells were co-transfected with pcDNA3/EGFP-JMJD8 3’UTR or 3’UTR-mut and pri-miR-873-5p or ASO-miR-873-5p, and the EGFP intensity was detected via spectrophotometer, which showed that JMJD8 is a direct target of miR-873-5p. d–e In HCT116 and SW480 cells transfected with pri-miR-873-5p or ASO-miR-873-5p and respective controls, JMJD8 mRNA levels and protein expression were measured by RT-qPCR and Western blot. f The correlation analysis of experiments data demonstrated negative correlation between the expression level of miR-873-5p and JMJD8 mRNA level. g Kaplan-Meier survival curves showed that JMJD8 expression in colorectal cancer patients. All the experiments were repeated more than three times. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS, not significant