Fig. 5.

miR-873-5p inhibits NF-ΚB pathway in CRC. a NF-κB reporter vector and indicated plasmids were cotransfected into HCT116 and SW480 cells, respectively, after which luciferase activity was measured. b–c RT-qPCR was used to examine the level of JMJD8, P65 in HCT116 and SW480 cells. d–e Western blot were used to detect the protein levels of JMJD8 and NF-κB (p65) in the total, cytosol and nuclear cellular fractions of HCT116 and SW480 cells. f–g Western blot were used to detect the protein p65 in the total, cytosol and nuclear cellular fractions of HCT116 and SW480 cells. h–i Western blot confirmed the protein level of NF-κB(p65, p-p65), EMT (E-cadherin, Vimentin) and the anti-apoptosis factors level including BCL-2, BCL-XL and survivin in HCT118 and SW480 cells. J Proposed model showed that miR-873-5p supresses tumorigenesis by targeting JMJD8 and inhibiting NF-κB pathway. All the experiments were repeated more than three times. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS, not significant