Fig. 1.

Early thrombin mediated linker region phosphorylation is through the transactivation EGFR. VSMCs were treated with thrombin for 30 min, EGF for 15 min or TGF-β for 30 min, in the presence and absence of A-B EGFR antagonist AG1478 (0.5-10 μM) or C-D TGFBR1 antagonist SB431542 (0.3-30 μM). Blots were probed with antibodies specific to A,C. phospho-Smad2/3(Thr220/179) or B,C. phospho-Smad2(Ser245/250/255). Blots are representative of three-independent experiments. Histogram represents band density expressed as fold per basal. Statistical significance was determined by one-way ANOVA, followed by least significant difference post-hoc analysis ##,p < 0.01 versus basal and **, p < 0.01 agonist versus antagonist