Fig. 5.

Thrombin mediated mRNA expression of GAG synthesizing genes CHST11 and CHSY1 are dependent on MMP activation and an intact cytoskeleton. VSMCs were pre-incubated with treated MMP inhibitor, GM6001 (10 μM) and ROCK inhibitor Y27632 (10 μM) and then exposed to thrombin for 6 h to study the effects on the mRNA expression of a. CHST11 and b. CHSY1. Data are expressed as the means ± standard error from three experiments. Statistical significance was determined by one-way ANOVA, followed by least significant difference post-hoc analysis. ##p < 0.01 basal versus thrombin and *p < 0.05 thrombin versus inhibitor was used for statistical analysis