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VSV deep sequencing. (A) Nonsynonymous mutation frequencies along the viral genome. Mutation frequencies (no. of mutated reads/no. of total base reads, excluding indels) were calculated by pooling all reads of lineages of the same treatment (aggregated versus nonaggregated). (B) Nonsynonymous polymorphisms at >0.1% in frequency found in VSV genes in the founder virus and evolved lines.
