FIG 4.

In vivo interactions between C. jejuni MS ring and C-ring proteins. N- or C-terminal FLAG-tagged proteins were expressed from plasmids in respective C. jejuni mutants and immunoprecipitated by FLAG tag antibody resin after cross-linking cells by formaldehyde. The position of the FLAG tag is indicated by the position of the asterisk (*) at the beginning or end of each protein that labels each lane at top. Each lane shows the detected proteins that coimmunoprecipitated with the FLAG-tagged protein indicated. Each protein was detected with specific antiserum. A whole-cell lysate (WCL) of WT C. jejuni was run alongside the coimmunoprecipitated proteins to indicate the size and position of the native protein. Arrows indicate the correct size of the monomer for each protein detected in the immunoblot. Arrowheads indicate bands that may represent a complex formed by the FLAG tag-immunoprecipitated protein with other proteins.