FIG 5.

CARD9-deficient macrophages are unable to effectively kill cryptococci. (A to D) Pulmonary DCs (A and B) or macrophages (C and D) were isolated from C57BL/6 and CARD9-deficient mice on day 7 postinoculation and immediately processed to quantify fungal burden or cultured ex vivo for 24 h and then lysed with endotoxin-free deionized water to enumerate the internalized cryptococci. Data shown are the means ± SEM and are cumulative of five independent experiments using three or five mice per group. Statistical significance by an unpaired Student’s t test (two-tailed test) is indicated as follows: ****, P < 0.0001; ns, not significant.