FIG 5.

Circular versus linear vIRF4 RNA decay rates. BCBL1 cells were treated with 2 μM flavopiridol, 1 × 10⁷ cells were collected every hour for 6 h, and qRT-PCR was performed using a TaqMan probe spanning the circRNA junction and a probe corresponding to the 3′ end of the linear transcript outside the circRNA coding sequence. (A) vIRF4. (B) A cellular control (SMARCA5). RNase P was used as a control that was not affected by RNA Pol II inhibition. Error bars show means ± SD of results from three replicates from 3 independent experiments.