Figure 4.

Conjugation of capture antibodies onto nanofiber-embedded substrates enables highly efficient CTC enrichment. a) TiNF-embedded substrates were prepared via electrospinning deposition of horizontally oriented precursory nanofibers, followed by calcination and anti-EpCAM conjugation. Fluorescent micrographs (bottom left) and SEM images (bottom right) suggest that the resulting TiNF-embedded substrates demonstrated a higher CTC capture efficiency and affinity than that observed for flat silicon substrates, respectively. Reproduced with permission.^[122] Copyright 2012, John Wiley and Sons. b) Schematic representation of a PN-NanoVelcro Chip consisting of an overlaid PDMS chaotic micromixer and a transparent PN-NanoVelcro substrate. i) Biotinylated anti-EpCAM (a prostate cancer-specific capture agent), reproduced with permission.^[163] Copyright 2013, John Wiley and Sons or ii) biotinylated anti-CD146 (a melanoma-specific capture agent) were covalently conjugated on the PN-NanoVelcro substrates, Reproduced with permission.^[129] Copyright 2013, John Wiley and Sons.