Skip to main content
. Author manuscript; available in PMC: 2020 May 4.
Published in final edited form as: Nat Methods. 2019 Nov 4;17(1):107–113. doi: 10.1038/s41592-019-0625-2

Fig. 1. Design of a dual-axis microscope for simultaneous mesoscopic and two-photon imaging.

Fig. 1.

a, Schematic overview of the dual-axis microscope. Left insets show the position of the two-photon objective relative to an implanted glass microprism and titanium headpost. Upper right inset shows timing of the widefield LED illumination, widefield sCMOS detector, two-photon excitation laser, and two-photon galvanometric Y-scan mirror. b, Example frames showing two-photon imaging (left) and mesoscopic imaging under blue (middle) and violet (right) illumination. Scale bar is 20 μm (left) and 2 mm (middle). c, Example cellular (orange) and mesoscopic (blue, violet) activity traces from the color-coded regions/cells shown in (b).