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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2019 Sep 23;19(1):221–230. doi: 10.1158/1535-7163.MCT-19-0103

Figure 2.

Figure 2.

GSH de novo synthesis support cellular physiology in IDH1-mutated cells

A. Western blot measures the expression of GSH synthesis enzymes in U251 cells with IDH1 mutant expression. β-actin was used as internal control.

B. Quantitative real-time PCR analysis measures mRNA level of GSH synthesis enzymes. *p<0.05, **p<0.01.

C. GSH and GSSG level was measured in IDH1-mutated U251 cells. Catalase (Cata) was used as exogenous ROS scavenger (500U/mL, 24 hr). **p<0.01.

D. Annexin V/PI apoptotic analysis in IDH1-mutated U251 cells with genetic silencing of GCLC and GCLM.

E. Quantification of apoptotic cells in D.

F. ROS-Glo assay in IDH1-mutated U251 cells with genetic silencing of GCLC and GCLM.