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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Virology. 2019 Jul 8;535:179–188. doi: 10.1016/j.virol.2019.07.008

Figure 6. Mucosal IgG and antibody-secreting plasma cells in draining lymph nodes after N1 VLP vaccination and rgAH5N1 virus challenge.

Figure 6.

(A) IgG antibodies specific for A/Cal (H1N1) virus in mucosal BALF and lung samples collected at 7 dpi with rgH5N1 challenge. (B-C) IgG antibody production from in vitro cultures with MLN cells collected at 7 dpi. MLN day 2 culture in vitro-secreted IgG antibodies specific for A/Cal (H1N1) virus (B) and for rgH5N1 virus (C). (D) Total mature B cells (IgD−CD19+ B cells) in MLN cells collected at 7dpi. (E and F) Germinal center B cells (GL7+B220+ B cells) and plasma cells (CD138+B220+/− B cells) in MLN cells collected at 7dpi were determined from gated IgD−CD19+B220+ B cells by flow cytometry. Data (n=4) are representative of individual animal out of two independent experiments. Error bars indicate the means ± SEM. *, p<0.05, **, p<0.01, ***, p<0.001.