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Specific primer sequences for real-time quantitative polymerase chain reaction
| Primer name | Primer sequences(5′–3′) |
|---|---|
| TCONS_00010987 | F: GGTTTTCACACAGAACAATCAGG |
| R: GACCAGCCAAAGGGTATCAA | |
| LEPR | F: ATTCCTGATTCCGTGGTGAA |
| R: CGGGAGACTGGTGGATTT | |
| miR-323 | F: CTGCACATTACACGGTCGACCT |
| GAPDH | F: GGAGAACGGGAAGCTTGTCA |
| R: GGTTCACGCCCATCACAAAC | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT |
LEPR, leptin receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
