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. 2019 Aug 23;33(2):360–372. doi: 10.5713/ajas.19.0480

Figure 1.

Figure 1

Generation of GGTA1 knockout Yucatan miniature pigs. (A) Schematic diagrams of targeting pig GGTA1 gene by homologous recombination (up) or by TALEN (down). Homologous recombination knockout vector contained 2.5 Kb left arm, knockout plasmid, and 4.0 Kb left arm. TALEN vector contained 20-mer TALE at each side (underlined). (B) Sequencing results of TALEN-mediated GGTA1 knockout pigs. Underlined sequences in wild type control (WT) indicate TALE sequences, gray characters indicate target sequence. Red characters indicate inserted sequences, and blue deleted sequences in TALEN-treated pigs (CB1 and CB3). (C) Expression analyses of GGTA1 protein in F1 generation of TALEN-treated pigs by flow cytometry. Red lines indicate GGTA1 expression in TALEN-treated pigs, black lines indicate those in wild type strain control. GGTA1, α-1,3-galactosyltransferase; TALEN, transcription activator-like effector nuclease.