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. 2019 Dec 2;8(12):2115. doi: 10.3390/jcm8122115

Figure 3.

Figure 3

CRISPR/Cas9 mediated knockout of miR-23b or miR-27b gene sensitized HCT-Oxa-c cells to Oxa. (A). Mutations in the hsa-miR-23b and hsa-miR-27b genes were identified in both alleles. 7 nt and large 634 nt deletion were determined in miR-23b gene. The 2bp indel was detected in a seed miR-27b-3p sequence. Seed sequences are marked in bold, deletions in red. (B) Mutations significantly inhibit the expression of miR-23b-3p, miR-27b-3p and miR-27b-5p. Data normalized compared to HCT-Oxa-c. Relative expression is presented as the average ± SD of three biological experiments. ***, p ≤ 0.001. (C) Mutations in the miR-23b gene leads to considerable increased HCT-Oxa-c sensitivity to Oxa. miR-27b depletion caused a slight impact to cell viability. Cell viability was determined with the MTT test.