Figure 4.

Wnt/β-catenin signaling was significantly activated in cell culture for 6 days. Cells were seeded and cultured in 6-well plates. Protein and RNA were harvested at the indicated time point, as 0 day, 2 day, 4 day, 6 day, 8 day. A, B. The mRNA and protein levels of β-catenin, CK16, CK18, Ki67 and PCNA were analyzed using RT-qPCR and western blotting respectively, and normalized by GAPDH expression. Data represent mean ± SD of three replicates. *indicates significant difference at P<0.05. **Indicates significant difference at P<0.01.