Figure 5.

Dkk1 suppressed cell proliferation and Wnt/β-catenin signaling in cells. A. Cells were seeded and cultured in 96-well plates. Cell viability was assayed using a CCK8 kit according to the manufacturer’s instruction at indicated time points. Overexpression of Dkk1 suppressed cell proliferation. B, C. qRT-PCR and western blotting showed that overexpression of Dkk1 in cholesteatoma cells repressed Wnt/β-catenin signaling, the expression levels of β-catenin, CK16, CK18, Ki67 and PCNA were down-regulated in cholesteatoma cells. The density of protein levels of above was quantified by ImageJ software and normalized to the level of GAPDH. Data represent mean ± SD of three replicates. *Indicates significant difference at P<0.05.