Figure 6.

Overexpression of Wnt3a induced cell proliferation and Wnt/β-catenin signaling in cells. A. Cells were seeded and cultured in 96-well plates. Cell viability was assayed using a CCK8 kit according to the manufacturer’s instruction at indicated time point. Overexpression of Wnt3a promoted activity of cell proliferation. B, C. qRT-PCR and western blotting showed that overexpression of Wnt3a in cholesteatoma cells promoted Wnt/β-catenin signaling, the expression levels of β-catenin, CK16, CK18, Ki67 and PCNA were down-regulated in cholesteatoma cells. The densities of protein levels of the above were quantified by ImageJ software and normalized to the level of GAPDH. Data represent mean ± SD of three replicates. *Indicates significant difference at P<0.05. **Indicates significant difference at P<0.01.