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. 2019 Apr 1;12(4):1272–1283.

Figure 4.

Figure 4

miR-195 regulated the apoptosis and autophagy of LNCaP cells by targeting CCNE1. A, B: qPCR and western blot showed the expression of CCNE1 in LNCaP cells after transfection with pcDNA-CCNE1, pcDNA3.1, si-CCNE1, and si-NC. C: Flow cytometry showed LNCaP cell apoptosis in different transfected groups. D: Western blot showed the expression of apoptosis-related proteins in different transfected groups. E: qPCR and western blot showed the expression of autophagy markers in different transfected groups. F: The expression of PI3K/AKT/mTOR pathway-related proteins after suppression of SNHG12, miR-195 and/or CCNE1. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to their respective controls.