Figure 2.

Gpx3 suppressed GC cell migration, invasion, and metastasis. A. Protein expression of Gpx3 was detected in AGS cells when overexpressed with Gpx3 by using western blot. B. The protein expression of Gpx3 was detected in SGC-7901 cells when transfected with the specific siRNA of Gpx3 by using western blot. C. Cell migration and invasion of AGS cells was analyzed after overexpression of Gpx3 for 72 h by using transwell chamber assay, respectively. D. The cell migration and invasion of SGC-7901 cells after transfection with the specific siRNA of Gpx3 for 72 h by using transwell chamber assay, respectively. E. AGS cells overexpressing Gpx3 were i.v. injected into NCG animals (n=6 per group). The lung metastatic loci were counted and lung sections were subjected to H&E staining. “**” denotes P < 0.01. “***” denotes P < 0.001. Data are expressed as the mean ± SD.