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Overexpression of FOXO1 inhibits β cell survival. Cells were transfected with pcDNA-FOXO1. A, B. The expression of FOXO1 was detected by qRT-PCR and western blot. C. Flow cytometry analyzed cell apoptosis. D. EDU staining tested cell proliferation. E. Western blot assessed PCNA, Bcl-2, p27, Bax, and cleaved caspase-3 expression. **indicates P<0.01, ***indicates P<0.001.
