Figure 2.

PQ401 inhibits U2OS and 143B cell proliferation. A. U2OS cell cells were treated with PQ401 (0.25 to 20 µM) for 48 h followed by application of the MTT assay. Culture medium was used as the blank control and indicated as 0 µM. Cisplatin was used as positive control and applied at indicated concentrations. B. PQ401 effect on 143B cell line. C. Percentage of cell viability of U2OS and 143B cell lines was significantly decreased with PQ401 treatment at a concentration of 5 µM at the indicated time point compared with the culture medium control group. At 24, 48, and 72 h, the cell viability was assessed. D. Real-time PCR for the relative IGF1R expression between the two selected cell lines. The means and error bars (standard deviation) were derived from experiments using triplicate samples. (***P<0.001).