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miR-221 positively regulates ISGs expression. SiHa cells were transfected with miR-221 mimics or inhibitor for 24 h, followed treatment using the calcium phosphate protocol with E2, E1 and pOri plasmids. Expression level of MxA (A, D), OAS (B, E) and ISG15 (C, F) were examined by qRT-PCR assay. Data represent the mean ± SD of three independent experiments. **P < 0.01 vs. mimics NC or inhibitor NC.
