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. 2019 May 1;12(5):1565–1577.

Figure 2.

Figure 2

MiR-92a interacts with the 3’-UTR of RECK mRNA. (A) Bioinformatic analysis identified the miR-92a binding site in the 3’-UTR of RECK mRNA, which is located at the 1357-1363 region and is highly conserved in different species. (B) The level of miR-92a expression in six CRC cell lines. (C, D) HCT116 (C) and SW620 (D) cell lines were cotransfected with the RECK gene 3’-UTR reporter vector and miR-92a-mimic or miR-92a-inhibitor. Luciferase activity was determined and normalized to Renilla luciferase activity. Data represent the average of three independent experiments. Mean ± SD, P<0.05 was considered statistically significant. Abbreviations: RECK, reversion-inducing cysteine-rich protein with Kazal motifs; 3’-UTR, the 3’-UTR reporter plasmid of the RECK gene; mimics, miR-92a mimics; inhibitor, miR-92a inhibitor; NC, negative control.