Figure 3.

RECK is the direct target of miR-92a in CRC. (A) IHC staining of RECK protein in 21 pairs of CRC and nontumorous tissues. The expression of RECK protein was lower in CRC samples than in adjacent nontumorous tissues. (B) The expression of RECK protein was negatively correlated with the expression of miR-92a in CRC (R²=0.737; P<0.01). (C, D) After transfection with miR-92a mimics, the expression level of miR-92a increased in HCT116 (C) and SW620 (D) cell lines, while the expression of RECK protein decreased. Conversely, after transfection with miR-92a inhibitor, the opposite trend was achieved. (E, F) The expression of RECK mRNA was detected by qRT-PCR, and there was no significant change in RECK mRNA level after transfection. Abbreviations: RECK, reversion-inducing cysteine-rich protein with Kazal motifs; NC, negative control; IHC, immunohistochemistry.