Figure 4.

MiR-92a may promote the invasion and migration of CRC cells via the RECK-MMP signaling pathway. (A) HCT116 and SW620 cell lines were transfected with miR-92a mimics, and the expression of RECK protein was upregulated compared with that of the negative control and blank control groups, while the MMP2 and MMP9 protein levels were downregulated. Conversely, after transfection with miR-92a inhibitor, the opposite trend was achieved. (B, C) The status of cell invasion and migration was determined by transwell assays and wound healing assays. Cell invasion (B) and migration (C) were increased in cells treated with miR-92a mimics, whereas cell invasion (B) and migration (C) were decreased in cells treated with miR-92a inhibitor. Abbreviations: RECK, reversion-inducing cysteine-rich protein with Kazal motifs; NC, negative control.